- Gaussian blur sigma=2, convert to frequencies before pixel FlowSOM, normalize including calibration channel
- Pixel hClusters found using z-score cap=3, k=20, and iterative thresh=2 (1 iteration)
- Cell-based clustering using frequencing of 20 hClusters (cap): normalize by size
- k=10 for cell clustering
Cell-based FlowSOM clustering using 100 pixel clusters
Cell-based FlowSOM clustering using hClusters (cap, k=20)